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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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PLEKHA4 Antibody (aa504‑553) LS‑C101976
PLEKHA4 antibody LS-C101976 is an unconjugated rabbit polyclonal antibody to PLEKHA4 from human, mouse, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C101976-50
50 µg
Unavailable

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Product Description

PLEKHA4 antibody LS-C101976 is an unconjugated rabbit polyclonal antibody to PLEKHA4 from human, mouse, guinea pig and other species. Validated for WB.
About PLEKHA4
Binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P), but not to other phosphoinositides. Q9H4M7 NM_020904 NP_065955.2

PLEKHA4 Antibody, PEPP1 Antibody, PEPP-1 Antibody


Specifications

Target
Human PLEKHA4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Dog, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PLEKHA4 antibody was raised against synthetic peptide located between aa504-553 of human PLEKHA4 (Q9H4M7, NP_065955). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%); Dog, Bovine, Bat, Rabbit, Pig (92%); Galago, Mouse, Elephant, Horse (85%); Guinea pig (84%).
Blocking Peptide
LS-E15678 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101976. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa504-553
Specificity
Human PLEKHA4
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLEKHA4 antibody LS-C101976 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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