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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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PLD2 / Phospholipase D2 Antibody (aa467‑516) LS‑C101426
Phospholipase D2 antibody LS-C101426 is an unconjugated rabbit polyclonal antibody to Phospholipase D2 (PLD2) from human, rat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C101426-50
50 µg
Unavailable

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PLD2 antibody LS-C101680 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

Phospholipase D2 antibody LS-C101426 is an unconjugated rabbit polyclonal antibody to Phospholipase D2 (PLD2) from human, rat, bovine and other species. Validated for WB.
About PLD2 / Phospholipase D2
PLD2 / Phospholipase D2 catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene. O14939 NM_002663 NP_002654.3

PLD2 Antibody, Choline phosphatase 2 Antibody, Phospholipase D2 Antibody, HPLD2 Antibody, PLD 2 Antibody, PLD1C Antibody


Specifications

Target
Human PLD2 / Phospholipase D2
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bovine (tested or 100% immunogen sequence identity)
Predicted
Bat, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa467-516 of human PLD2 (O14939, NP_002654). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bovine (100%); Bat, Pig (91%); Rat (84%).
Blocking Peptide
LS-E11637 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101426. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa467-516
Specificity
Human PLD2
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PLD2 antibody LS-C101426 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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