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Anti-PLCG1 Antibody (phospho-Tyr783) LS-C9603

Catalog	Size	Price
LS-C9603-200	200 µg	Unavailable

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Western blot of recombinant PLCG1. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Antibody:	Rabbit Polyclonal (IgG) to Human PLCG1
Reactivity:	Human, Mouse, Rat, Bovine, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human PLCG1
Host:	Rabbit
Reactivity:	Human, Mouse, Rat, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:	PLCG1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa surrounding phospho-Tyr783 of human PLCg-1.
Epitope:	pTyr783
Specificity:	Recognizes PLCg-1 phosphorylated on Tyr783, Mr ~135kD. Species cross-reactivity: Human and mouse. Cross-reactivity with rat, rabbit and bovine expected due to sequence homology.
Usage:	Suitable for use in Western Blot. Western Blot Analysis: 1-2 ug/ml of this lot detected phospho-PLCg-1 in RIPA lysates from EGF-stimulated A431 cells.

Packaging

Presentation:	0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage:	Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About PLCG1

Uniprot: P19174 NCBI: NM_002660 NP_002651.2

Synonyms: PLCG1 Antibody, Phospholipase C, gamma 1 Antibody, Phospholipase C-148 Antibody, Phosphoinositidase C Antibody, Phospholipase C-II Antibody, Inositoltrisphosphohydrolase Antibody, PLC-gamma-1 Antibody, PLC1 Antibody, PLC-148 Antibody, PLC-II Antibody, PLC148 Antibody, NCKAP3 Antibody, Phospholipase C-gamma-1 Antibody, PLCgamma1 Antibody

Description: PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras.

(More About PLCG1)

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