Bovine, Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.1 - 0.5 µg/ml)
- (applications tested for the base form of this product only)
PLCG1 antibody was raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
Recognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins. Species cross-reactivity: rabbit, rat, mouse and human.
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Western Blot: 0.1-0.5 ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts. Immunoprecipitation: 4 ug (bovine brain cytosol).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.