Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
PLCG1 antibody was raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
Recognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins. Species cross-reactivity: rabbit, rat, mouse and human.
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Western Blot: 0.1-0.5 ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts. Immunoprecipitation: 4 ug (bovine brain cytosol).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras.