Target
Bovine PLCG1
Synonyms
PLCG1 | Phospholipase C, gamma 1 | Phospholipase C-148 | Phosphoinositidase C | Phospholipase C-II | Inositoltrisphosphohydrolase | PLC-gamma-1 | PLC1 | PLC-148 | PLC-II | PLC148 | NCKAP3 | Phospholipase C-gamma-1 | PLCgamma1
Reactivity
Bovine, Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Purified soluble phospholipase C (PLC) g-1 from bovine brain.
Specificity
Recognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins. Species cross-reactivity: rabbit, rat, mouse and human.
Applications
- Western blot (0.1 - 0.5 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Western Blot: 0.1-0.5 ug/ml. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts. Immunoprecipitation: 4 ug (bovine brain cytosol).
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee