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Anti-PLCG1 Antibody (C-Terminus) IHC-plus™ LS-B2311

Note: This antibody replaces LS-C9599

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.05 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PLCG1 Antibodies

Anti-PLCG1 Antibody (C-Terminus) IHC-plus™ LS-B2311
Rabbit Polyclonal (IgG) to Human PLCG1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PLCG1 Antibody (aa751-800) IHC-plus™ LS-B6987
Rabbit Polyclonal (IgG) to Human PLCG1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PLCG1 Antibody (clone 2B1) IHC-plus™ LS-B7825
Mouse Monoclonal [clone 2B1] (IgG2a,k) to Human PLCG1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PLCG1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human PLCG1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:25 - 1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)

Specificity and Use

PLCG1 antibody was raised against synthetic peptide (KLH coupled) corresponding to the C-terminal residues of human PLCgamma1.
C-Terminus
Recognizes endogenous levels of total human PLC gamma1 protein. Does not cross-react with PLCgamma2. Species cross-reactivity: mouse and rat. MW: 155kD.
Immunohistochemistry: LS-B2311 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2311 was determined to be 1:25-1:100.

Packaging

10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About PLCG1

P19174 NM_002660 NP_002651.2

PLCG1 Antibody, Phospholipase C, gamma 1 Antibody, Phospholipase C-148 Antibody, Phosphoinositidase C Antibody, Phospholipase C-II Antibody, Inositoltrisphosphohydrolase Antibody, PLC-gamma-1 Antibody, PLC1 Antibody, PLC-148 Antibody, PLC-II Antibody, PLC148 Antibody, NCKAP3 Antibody, Phospholipase C-gamma-1 Antibody, PLCgamma1 Antibody

PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras.

Immunohistochemistry

Immunohistochemistry
Anti-PLCG1 antibody IHC of human colon, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2311 dilution 1:25.
 

Requested From: United States
Date Requested: 12/3/2016

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