Target
Human PLA2G4F
Synonyms
PLA2G4F | CPLA2-zeta | Phospholipase A2 group IVF | Phospholipase A2, group IVF | PLA2G4FZ | PLA2G4F/Z
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QTAEEKAFGDFVINR, from the internal region (near C-Terminus) of the protein sequence according to NP_998765.3.
Specificity
Human PLA2G4F / CPLA2-zeta.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 55-60kD and 35kD in Human Placenta lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kD according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee