Target
Human PJA1 / PRAJA1
Synonyms
PJA1 | Praja ring finger 1 | PRAJA1 | Praja 1 | RNF70 | RING finger protein 70
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-HSTSRWRDTANDNE, from the internal region of the protein sequence according to NP_660095.1NP_001027568.1NP_071763.2.
Specificity
Human PJA1 / RNF70. This antibody is expected to recognize all reported isoforms (NP_660095.1; NP_001027568.1; NP_071763.2).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx 30kD band in Human Brain and Mouse Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.4kD according to NP_071763.2. The 30kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee