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Anti-PITX3 Antibody (N-Terminus) IHC-plus™ LS-B2606

Note: This antibody replaces LS-C17510

Ordering

Wt. Vol. Conc. Price
25 µg - 0.25 mg/ml $395

LSBio (Direct) LSBio (Direct)
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PITX3 Antibody LS-B2606 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PITX3 antibody IHC of human brain, cortex, white matter.

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Rabbit Polyclonal (IgG) to Mouse PITX3
Mouse, Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Mouse PITX3
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Immunofluorescence (0.7 - 2 µg/ml), Western blot (1 - 3 µg/ml)

Specificity and Use

PITX3 antibody was raised against synthetic peptide derived from the N-terminal region of the mouse PiTX3 protein.
N-Terminus
Recognizes mouse PiTX3 protein. Reactivity has been confirmed with fetal mouse brain homogenate.
Immunohistochemistry: LS-B2606 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2606 was determined to be 5 ug/ml. Western Blot: 1-3 ug/ml. Identifies a single band at ~32kD. Bands of unknown origin are observed at ~40 and 50kD.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About PITX3

O75364 NM_005029 NP_005020.1

PITX3 Antibody, CTPP4 Antibody, Homeobox protein PITX3 Antibody, Paired-like homeodomain 3 Antibody, Pituitary homeobox 3 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-PITX3 antibody IHC of human brain, cortex, white matter. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2606 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 7/28/2015

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