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Anti-PITX1 Antibody (clone 5G4) LS-C197909

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PITX1 Antibodies

Anti-PITX1 Antibody (aa50-100) LS-C91661
Rabbit Polyclonal to Human PITX1
Human
Western blot, Immunoprecipitation
Unconjugated
Anti-PITX1 Antibody (aa123-150) LS-C100923
Rabbit Polyclonal to Human PITX1
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5G4] (IgG2a,k) to Human PITX1
Human
Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human PITX1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5G4]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

PITX1 antibody was raised against pITX1 (NP_002644, aa225-313 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PITX1
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PITX1

P78337 NM_002653 NP_002644.4

PITX1 Antibody, BFT Antibody, Homeobox protein PITX1 Antibody, LBNBG Antibody, Paired-like homeodomain 1 Antibody, Pituitary otx-related factor Antibody, Pituitary homeo box 1 Antibody, Pituitary homeobox 1 Antibody, CCF Antibody, POTX Antibody, PTX1 Antibody

May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Western blot

Western blot
Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
PITX1 monoclonal antibody (M01), clone 5G4 Western Blot analysis of PITX1 expression in HeLa NE.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PITX1 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
 

Requested From: United States
Date Requested: 12/5/2016

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