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Anti-PIM2 / Pim-2 Antibody (aa277-308) LS-C99724

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PIM2 / Pim-2 Antibodies

Anti-PIM2 / Pim-2 Antibody (aa282-311, FITC) LS-C216776
Rabbit Polyclonal (IgG) to Human PIM2 / Pim-2
Human
IHC, Western blot, Immunoprecipitation, ELISA
FITC Conjugated
Anti-PIM2 / Pim-2 Antibody (aa287-299) IHC-plus™ LS-B10470
Goat Polyclonal to Human PIM2 / Pim-2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PIM2 / Pim-2 Antibody (aa23-36) IHC-plus™ LS-B10471
Goat Polyclonal to Human PIM2 / Pim-2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PIM2 / Pim-2
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human PIM2 / Pim-2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:50 - 1:100)

Specificity and Use

PIM2 / Pim-2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 277-308 from C-terminal region of human PIM2.
LS-E9380 - Lyophilized - 100 µg - $145.00
aa277-308

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About PIM2 / Pim-2

Q9P1W9 NM_006875 NP_006866.2

PIM2 Antibody, DXCch3 Antibody, Pim-2 oncogene Antibody, Pim-2h Antibody, Tic-1 Antibody, Proviral integration site 2 Antibody

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
Western blot of anti-PIM2 Antibody in HeLa cell line lysates (35 ug/lane). PIM2 (arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.

Immunoprecipitation

Immunoprecipitation
PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.

Requested From: United States
Date Requested: 12/3/2016

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