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Catalog Number Size Price
LS-C99724-400 400 µl $393 
PIM2 / Pim-2 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIM2 / Pim-2 Antibody - Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
PIM2 / Pim-2 Antibody - Western blot of anti-PIM2 Antibody in HeLa cell line lysates (35 ug/lane). PIM2 (arrow) was detected using the purified antibody.
PIM2 / Pim-2 Antibody - PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
PIM2 / Pim-2 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIM2 / Pim-2 Antibody - Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
PIM2 / Pim-2 Antibody - Western blot of anti-PIM2 Antibody in HeLa cell line lysates (35 ug/lane). PIM2 (arrow) was detected using the purified antibody.
PIM2 / Pim-2 Antibody - PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
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Polyclonal Rabbit anti‑Human PIM2 / Pim‑2 Antibody (aa277‑308, IHC, WB) LS‑C99724

Polyclonal Rabbit anti‑Human PIM2 / Pim‑2 Antibody (aa277‑308, IHC, WB) LS‑C99724

Antibody:
PIM2 / Pim-2 Rabbit anti-Human Polyclonal (aa277-308) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C99724-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PIM2 / Pim-2 Rabbit anti-Human Polyclonal (aa277-308) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Pim-2 antibody LS-C99724 is an unconjugated rabbit polyclonal antibody to human Pim-2 (PIM2) (aa277-308). Validated for IHC, IP and WB.
Target
Human PIM2 / Pim-2
Synonyms
PIM2 | DXCch3 | Pim-2 oncogene | Pim-2h | Tic-1 | Proviral integration site 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa277-308
Specificity
This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIM2 / Pim-2
Q9P1W9 NM_006875 NP_006866.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024