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Anti-PIM2 / Pim-2 Antibody (aa277-308) LS-C99724

Catalog Size Price
LS-C99724-400 400 µl Unavailable

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60 PIM2 / Pim-2 Antibodies

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100% Guaranteed
Rabbit Polyclonal to Human PIM2 / Pim-2
Human
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human PIM2 / Pim-2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:50 - 1:100)

Specificity and Use

LS-E9380 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99724. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa277-308
This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About PIM2 / Pim-2

Q9P1W9 NM_006875 NP_006866.2

PIM2 Antibody, DXCch3 Antibody, Pim-2 oncogene Antibody, Pim-2h Antibody, Tic-1 Antibody, Proviral integration site 2 Antibody

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of anti-PIM2 Antibody in HeLa cell line lysates (35 ug/lane). PIM2 (arrow) was detected using the purified antibody.
Western blot of anti-PIM2 Antibody in HeLa cell line lysates (35 ug/lane). PIM2 (arrow) was detected using the purified antibody.

Western blot

Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
Western blot of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.

Immunoprecipitation

PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.

Requested From: 
Date Requested: 5/21/2018

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