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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PIK3CA / PI3K Alpha Antibody (clone 4F3) LS‑C169164
PI3K Alpha antibody LS-C169164 is an unconjugated mouse monoclonal antibody to human PI3K Alpha (PIK3CA). Validated for ELISA and Flow.
Catalog
Size
Price
LS-C169164-100
100 µg (1 mg/ml)
Unavailable

Popular PIK3CA / PI3K Alpha Products

Anti-PIK3CA antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2451 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Anti-PIK3CA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5363 concentration 5 ug/ml.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Anti-PIK3CA / PI3K antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

PI3K Alpha antibody LS-C169164 is an unconjugated mouse monoclonal antibody to human PI3K Alpha (PIK3CA). Validated for ELISA and Flow.
About PIK3CA / PI3K Alpha
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. P42336 NM_006218 NP_006209.2

PIK3CA Antibody, MCAP Antibody, p110-alpha Antibody, p110alpha Antibody, PI3K Antibody, PI3-kinase p110 subunit alpha Antibody, PI3-kinase subunit alpha Antibody, PI3Kalpha Antibody, MCM Antibody, MCMTC Antibody, PI3K-alpha Antibody, PtdIns-3-kinase subunit alpha Antibody, CLOVE Antibody


Specifications

Target
Human PIK3CA / PI3K Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4F3]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Immunogen
Purified recombinant fragment of human PIK3CA expressed in E. Coli.\
Specificity
Human
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunofluorescence

Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)
Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/24/2018

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