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Anti-PIK3CA / PI3K Alpha Antibody (clone 4F3) LS-C169164

Catalog Size Price
LS-C169164-100 100 µg (1 mg/ml) Unavailable

Most Popular PIK3CA / PI3K Alpha Antibodies

Anti-PIK3CA / PI3K Alpha Antibody (aa878-891) IHC-plus™ LS-B2451
Goat Polyclonal to Human PIK3CA / PI3K Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PIK3CA / PI3K Alpha Antibody (Internal) IHC-plus™ LS-B5363
Rabbit Polyclonal (IgG) to Human PIK3CA / PI3K Alpha
Human, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PIK3CA / PI3K Alpha Antibody (clone 4F3) LS-C169164
Mouse Monoclonal [clone 4F3] (IgG1) to Human PIK3CA / PI3K Alpha
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunofluorescence Image
Anti-PIK3CA / PI3K Alpha Antibody IHC-plus™ LS-B8751
Rabbit Polyclonal (IgG) to Mouse PIK3CA / PI3K Alpha
Mouse, Human, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PIK3CA / PI3K Alpha Antibody (Tyr317, FITC) LS-C280857
Rabbit Polyclonal (IgG) to Human PIK3CA / PI3K Alpha
Human
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed
Mouse Monoclonal [clone 4F3] (IgG1) to Human PIK3CA / PI3K Alpha
Human
ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human PIK3CA / PI3K Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4F3]
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

Purified recombinant fragment of human PIK3CA expressed in E. coli.
Human PIK3CA / PI3K

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PIK3CA / PI3K Alpha

P42336 NM_006218 NP_006209.2

PIK3CA Antibody, MCAP Antibody, p110-alpha Antibody, p110alpha Antibody, PI3K Antibody, PI3-kinase p110 subunit alpha Antibody, PI3-kinase subunit alpha Antibody, PI3Kalpha Antibody, MCM Antibody, MCMTC Antibody, PI3K-alpha Antibody, PtdIns-3-kinase subunit alpha Antibody, CLOVE Antibody

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HeLa cells using PIK3CA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using PIK3CA monoclonal antibody against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot

Western blot
Western blot using PIK3CA monoclonal antibody against human PIK3CA (AA: 881-1068) recombinant protein. (Expected MW is 47.4 kDa)

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 8/18/2017

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