Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
PIK3C3 / VPS34 antibody was raised against synthetic peptide made to an internal portion of the human protein (within residues 700-850). [Swiss-Prot# Q8NEB9]. Percent identity by BLAST analysis: Xenopus (87%).
PBS, 0.1% BSA, 50% glycerol, 0.05% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
PIK3C3 Antibody, HVps34 Antibody, Phosphatidylinositol 3-kinase Antibody, VPS34 Antibody, PI3-kinase type 3 Antibody, PI3K type 3 Antibody, PtdIns-3-kinase type 3 Antibody
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
VPS34 Antibody - cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY PIK3C3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3C3.