Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Guinea pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
Specificity and Use
PIGO antibody was raised against synthetic peptide located between aa74-123 of human PIGO (B1AML3, NP_690577). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Guinea pig (100%); Galago, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse (92%); Pig (85%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.