Human, Monkey, Guinea pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
Specificity and Use
PIGO antibody was raised against synthetic peptide located between aa74-123 of human PIGO (B1AML3, NP_690577). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Guinea pig (100%); Galago, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse (92%); Pig (85%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.