Clonality
IgG2b
Monoclonal
Conjugations
Horseradish Peroxidase.
Also available conjugated with
Biotin,
Biotin,
FITC,
FITC,
PE.
Purification
Protein A purified
Immunogen
Phosphotyramine-KLH.
Specificity
Recognizes human Phosphotyrosine.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
- Applications tested for the base form of this product only
Usage
Suitable for use in Western Blot. For use in applications in which an HRP conjugate is advantageous, such as Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml, with optimal performance at 2 ug/ml (10 ul per 10 ml blocking. buffer). Detects tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human. A431 carcinoma cells. EGF-stimulated cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Phosphotyrosine, HRP conjugate (1 ug/ml). Proteins were visualized using a chemiluminescence detection system. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.01% merthiolate. Sufficient for 10 immunoblots, if used at the optimum concentration of 2 ug/ml in 10 ml blocking buffer.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee