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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74935-10 | 10 Blots | $1,129 |
Monoclonal Mouse Phosphotyrosine Antibody (HRP, WB) LS‑C74935
Monoclonal Mouse Phosphotyrosine Antibody (HRP, WB) LS‑C74935
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Phosphotyrosine antibody LS-C74935 is an HRP-conjugated mouse monoclonal antibody to Phosphotyrosine. Validated for IP and WB.
Host
Mouse
Clonality
IgG2b
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Phosphotyramine-KLH.
Specificity
Recognizes human Phosphotyrosine.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
- Applications tested for the base form of this product only
Usage
Suitable for use in Western Blot. For use in applications in which an HRP conjugate is advantageous, such as Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml, with optimal performance at 2 ug/ml (10 ul per 10 ml blocking. buffer). Detects tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human. A431 carcinoma cells. EGF-stimulated cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Phosphotyrosine, HRP conjugate (1 ug/ml). Proteins were visualized using a chemiluminescence detection system. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.01% merthiolate. Sufficient for 10 immunoblots, if used at the optimum concentration of 2 ug/ml in 10 ml blocking buffer.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
