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Anti-Phosphotyrosine Antibody (AP) LS-C74888

Catalog Size Price
LS-C74888-100 100 µg Unavailable

Most Popular Phosphotyrosine Antibodies

Anti-Phosphotyrosine Antibody LS-C74917
Mouse Monoclonal (IgG2b)
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphotyrosine Antibody LS-C74946
Goat Polyclonal (IgG)
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphotyrosine Antibody (phospho-Tyr) LS-C76644
Mouse Monoclonal (IgG1)
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Anti-Phosphotyrosine Antibody LS-C90259
Mouse Monoclonal (IgG2b)
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphotyrosine Antibody (PE) LS-C131435
Mouse Monoclonal (IgG2b)
Flow Cytometry
PE Conjugated

100% Guaranteed
Mouse Monoclonal (IgG2b)
Western blot, Immunoprecipitation, ELISA
AP Conjugated

Details

Phosphotyrosine
Mouse
IgG2b Monoclonal
Alkaline Phosphatase
Purified
Unmodified

Applications

  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA

Specificity and Use

Phosphotyrosine antibody was raised against phosphotyrosine coupled to KLH.
Recognizes native and denatured tyrosine phosphorylated proteins Binding of this antibody is inhibited by phosphotyrosine. Will not react with phosphoserine or phosphothreonine residues on proteins. Species cross-reactivity: Human and mouse.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 2-10 ug/IP reaction. Note: Binding is inhibited by high salt concentrations and by divalent cations. Use a buffer with less than 150 mM sodium chloride and divalent cations not much greater than 1mM. Positive control: Mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Tris buffered saline, 1% BSA, 1 mM magnesium chloride, 0.05% sodium azide, 50% glycerol.
Store at 4°C. Do not freeze.
For research use only.

About Phosphotyrosine

Some of the tyrosine residues in protein can be modified (at the hydroxyl group) with a phosphate group (phosphorylated) by protein kinases. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies.

Requested From: 
Date Requested: 10/22/2017

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