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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PHF12 Antibody (aa866‑915) LS‑C82615
PHF12 antibody LS-C82615 is an unconjugated rabbit polyclonal antibody to PHF12 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C82615-50
50 µg
$365

Popular PHF12 Products

Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA

Product Description

PHF12 antibody LS-C82615 is an unconjugated rabbit polyclonal antibody to PHF12 from human, mouse, rat and other species. Validated for WB.
About PHF12
Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA. Q96QT6 AI092429 Q96QT6

PHF12 Antibody, KIAA1523 Antibody, PHD finger protein 12 Antibody, PF1 Antibody, PHD factor 1 Antibody


Specifications

Target
Human PHF12
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
PHF12 antibody was raised against synthetic peptide located between aa866-915 of human PHF12 (Q96QT6, NP_001028733). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%); Turkey, Chicken, Xenopus (85%).
Blocking Peptide
LS-E18202 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82615. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa866-915
Specificity
Human PHF12
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/19/2018

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