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Anti-PHB / Prohibitin Antibody (clone 3F4-2B2) IHC-plus™ LS-B5415

Note: This antibody replaces LS-C133382

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

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Most Popular PHB / Prohibitin Antibodies

Anti-PHB / Prohibitin Antibody (clone 3F4-2B2) IHC-plus™ LS-B5415
Mouse Monoclonal [clone 3F4-2B2] (IgG2a,k) to Human PHB / Prohibitin
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PHB / Prohibitin Antibody (aa222-272) LS-C286798
Rabbit Polyclonal (IgG) to Human PHB / Prohibitin
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image
Anti-PHB / Prohibitin Antibody (aa222-272) IHC-plus™ LS-B11330
Rabbit Polyclonal (IgG) to Human PHB / Prohibitin
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3F4-2B2] (IgG2a,k) to Human PHB / Prohibitin
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human PHB / Prohibitin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [3F4-2B2]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1 - 10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

PHB / Prohibitin antibody was raised against pHB (AAH13401, 1 a.a. ~ 273 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human Prohibitin
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PHB / Prohibitin

P35232 NM_002634 NP_002625.1

PHB Antibody, PHB1 Antibody, Prohibitin Antibody

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Immunohistochemistry

Immunohistochemistry
Anti-PHB / Prohibitin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5415 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~10 ug/ml].

Western blot

Western blot
PHB monoclonal antibody clone 3F4-2B2 Western blot of PHB expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PHB is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/8/2016

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