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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PHB / Prohibitin Antibody (aa100‑150) LS‑C203076
Prohibitin antibody LS-C203076 is an unconjugated rabbit polyclonal antibody to Prohibitin (PHB) from human, mouse and rat. Validated for ELISA and WB.
Catalog
Size
Price
LS-C203076-40
40 µg (0.5 mg/ml)
Unavailable

Popular PHB / Prohibitin Products

PHB / Prohibitin antibody LS-C29731 was used in IHC to stain formalin-fixed, paraffin-embedded human stomach.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-PHB / Prohibitin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5415 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-PHB / Prohibitin antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7282 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human Prohibitin by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Prohibitin used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence
Anti-PHB / Prohibitin antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11330 dilution 1:50.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

Prohibitin antibody LS-C203076 is an unconjugated rabbit polyclonal antibody to Prohibitin (PHB) from human, mouse and rat. Validated for ELISA and WB.
About PHB / Prohibitin
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. P35232 NM_002634 NP_002625.1

PHB Antibody, PHB1 Antibody, Prohibitin Antibody


Specifications

Target
Human PHB / Prohibitin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
Synthetic peptide (KLH-coupled) derived from within residues 100-150 of human prohibitin (Swiss Prot: P35232).
Epitope
aa100-150
Specificity
Endogenous levels of human, mouse, and rat prohibitin protein.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Reconstitution
80 µl Distilled water
Storage
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4°C or long term at -20°C or below. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD
Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD

Western blot

Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.
Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.

Western blot

Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD
Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD

Western blot

Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.
Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.

Western blot

Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD
Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD

Western blot

Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.
Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.

Western blot

Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

Western blot

Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD
Lane 1: Marker Lane 2 and 6: Ramos cell lysate Lane 3 and 7: HepG2 cell lysate Lane 4 and 8: Hela cell lysate Lane 5 and 9: NIH/3T3 cell lysate Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Prohibitin Polyclonal Antibody , without or with immunizing (blocking) peptide. The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD Observed band size: 30KD Additional band size: 38KD

Western blot

Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.
Lane 1: Marker. Lane 2 and 6: Ramos cell lysate. Lane 3 and 7: HepG2 cell lysate. Lane 4 and 8: HeLa cell lysate. Lane 5 and 9: NIH/3T3 cell lysate. Western blot of cell lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody, without or with immunizing (blocking) peptide. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted band size: 30KD. Observed band size: 30KD. Additional band size: 38KD.

Western blot

Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker. Lane 2: Mouse liver tissue lysate. Lane 3: Rat liver tissue lysate. Western blot of tissue lysates using 1 ug/ml Rabbit Anti-Prohibitin Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG.


Requested From: 
Date Requested: 6/22/2018

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