Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
IHC - Paraffin
Specificity and Use
PH4 / P4HTM antibody was raised against the epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human Prolyl Hydroxylase Domain-Containing Protein 4 using the numbering given in entry NP_808808.1 (GeneID 54681)
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Western blot of human PHD4. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: United States
Date Requested: 12/8/2016