Human PGBD1

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-EKWSHLSLTRRN, from the internal region of the protein sequence according to NP_115896.1.

aa230-241

Human PGBD1.

• Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave bands at approx 150kD and 17kD in lysates of cell lines HeLa, HepG2 and K562 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 92.5kD according to NP_115896.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96JS3 NCBI: NM_032507 NP_115896.1