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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PFK2 / PFKFB3 Antibody (aa454‑484) LS‑C101285
PFKFB3 antibody LS-C101285 is an unconjugated rabbit polyclonal antibody to human PFKFB3 (PFK2). Validated for IHC, Peptide-ELISA and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C101285-400
400 µl
Unavailable

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Product Description

PFKFB3 antibody LS-C101285 is an unconjugated rabbit polyclonal antibody to human PFKFB3 (PFK2). Validated for IHC, Peptide-ELISA and WB. Cited in 1 publication.
About PFK2 / PFKFB3
Synthesis and degradation of fructose 2,6-bisphosphate. Q16875 NM_004566 NP_004557.1

PFKFB3 Antibody, 6PF-2-K/Fru-2,6-P2ase 3 Antibody, IPFK2 Antibody, IPFK-2 Antibody, PFK/FBPase 3 Antibody, PFK2 Antibody


Specifications

Target
Human PFK2 / PFKFB3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E9530 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101285. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa454-484
Specificity
This PFKFB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-484 amino acids from the C-terminal region of human PFKFB3.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Ras transformation requires metabolic control by 6-phosphofructo-2-kinase. Telang S, Yalcin A, Clem AL, Bucala R, Lane AN, Eaton JW, Chesney J. Oncogene. 2006 25:7225-34. [PubMed:16715124]

Customer Reviews (0)


Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.
Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.
Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.
Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot

Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.
Western blot of anti-PFKFB3 Antibody in CEM cell line lysates (35 ug/lane). PFKFB3(arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/17/2018

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