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Anti-PEPD / PROLIDASE Antibody (clone 1D5-H3) LS-C197874

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PEPD / PROLIDASE Antibodies

Anti-PEPD / PROLIDASE Antibody (clone 1B7) IHC-plus™ LS-B11014
Mouse Monoclonal [clone 1B7] (IgG1) to Human PEPD / PROLIDASE
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PEPD / PROLIDASE Antibody (aa359-408) IHC-plus™ LS-B12732
Rabbit Polyclonal (IgG) to Human PEPD / PROLIDASE
Human, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1D5-H3] (IgG1,k) to Human PEPD / PROLIDASE
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PEPD / PROLIDASE
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1D5-H3]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

PEPD / PROLIDASE antibody was raised against pEPD (AAH15027, aa1-493 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PEPD / PROLIDASE
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PEPD / PROLIDASE

P12955 NM_000285 NP_000276.2

PEPD Antibody, Aminoacyl-L-proline hydrolase Antibody, Imidodipeptidase Antibody, PRD Antibody, PROLIDASE Antibody, X-Pro dipeptidase Antibody, Xaa-Pro dipeptidase Antibody, Peptidase D Antibody, Proline dipeptidase Antibody

PEPD / PROLIDASE is a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
PEPD monoclonal antibody (M01), clone 1D5-H3 Western Blot analysis of PEPD expression in HepG2.

Western blot

Western blot
Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PEPD is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/2/2016

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