Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
PEPD / PROLIDASE is a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline.
Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10 ug/ml]
PEPD monoclonal antibody (M01), clone 1D5-H3 Western Blot analysis of PEPD expression in HepG2.
Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody (M01), clone 1D5-H3.Lane 1: PEPD transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of PEPD transfected lysate using anti-PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PEPD is approximately 0.1 ng/ml as a capture antibody.