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PEBP1 / RKIP Antibody (N‑Terminus) LS‑C17571
RKIP antibody LS-C17571 is an unconjugated rabbit polyclonal antibody to RKIP (PEBP1) from human, mouse, bovine and other species. Validated for ELISA and WB.
100 µg

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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PBP monoclonal antibody (M01), clone 2G2-1F1. Western Blot analysis of PEBP1 expression in human kidney.
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Immunofluorescence analysis of HeLa cells using PEBP1 antibody. Blue: DAPI for nuclear staining.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, RNA Immunoprecipitation
Immunofluorescent analysis of RKIP staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

RKIP antibody LS-C17571 is an unconjugated rabbit polyclonal antibody to RKIP (PEBP1) from human, mouse, bovine and other species. Validated for ELISA and WB.


Human PEBP1 / RKIP
PEBP1, HCNP, HCNPpp, PBP, Prostatic binding protein, Raf kinase inhibitor protein, Raf kinase inhibitory protein, Prostatic-binding protein, Neuropolypeptide h3, PEBP, PEBP-1, RKIP
Human, Monkey, Mouse, Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Immunoaffinity purified
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
PEBP1 / RKIP antibody was raised against synthetic peptide derived from the N-terminal region of human RKIP (Raf kinase inhibitor protein).
Reacts with human and mouse RKIP. In Western Blots, identifies a single 23kD band. Reactivity has been confirmed with human HEK293 cell lysates and mouse brain homogenates. Based on amino acid sequence homology, expected to react with bovine and monkey RKIP.
Suitable for use in ELISA, Western Blot. ELISA: 0.1-1.0 ug/ml. Western Blot: 1-3 ug/ml.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
About PEBP1 / RKIP
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase By similarity. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. P30086 NM_002567 NP_002558.1

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Requested From: United States
Date Requested: 10/21/2018

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