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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PEBP1 / RKIP Antibody LS‑C17569
RKIP antibody LS-C17569 is an unconjugated rabbit polyclonal antibody to RKIP (PEBP1) from human, mouse and rat. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C17569-200
200 µg (1 mg/ml)
$695

Popular PEBP1 / RKIP Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-PEBP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3247 concentration 5 ug/ml.
Species: Human, Monkey, Bovine, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PBP monoclonal antibody (M01), clone 2G2-1F1. Western Blot analysis of PEBP1 expression in human kidney.
Species: Human
Applications: Western blot
Immunofluorescence analysis of HeLa cells using PEBP1 antibody. Blue: DAPI for nuclear staining.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, RNA Immunoprecipitation
Immunofluorescent analysis of RKIP staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

RKIP antibody LS-C17569 is an unconjugated rabbit polyclonal antibody to RKIP (PEBP1) from human, mouse and rat. Validated for IP and WB.
About PEBP1 / RKIP
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase By similarity. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. P30086 NM_002567 NP_002558.1

PEBP1 Antibody, HCNP Antibody, HCNPpp Antibody, PBP Antibody, Prostatic binding protein Antibody, Raf kinase inhibitor protein Antibody, Raf kinase inhibitory protein Antibody, Prostatic-binding protein Antibody, Neuropolypeptide h3 Antibody, PEBP Antibody, PEBP-1 Antibody, RKIP Antibody


Specifications

Target
Rat PEBP1 / RKIP
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
PEBP1 / RKIP antibody was raised against fusion Protein corresponding to full length rat RKIP (Raf kinase inhibitor protein).
Specificity
Recognizes RKIP, Mr 23kD. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects RKIP in RIPA cells. A previous lot detected RKIP in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 ug immunoprecipitates RKIP from 500 ug of 3T3/A31 RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/16/2018

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