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Anti-PDX1 Antibody (aa198-213) IHC-plus™ LS-B3086

Note: This antibody replaces LS-C74226
Catalog Size Price
LS-B3086-50 50 µg (0.5 mg/ml) Unavailable

Related Products

83 PDX1 Antibodies

Most Popular PDX1 Antibodies

Anti-PDX1 Antibody (aa198-213) IHC-plus™ LS-B3086
Rabbit Polyclonal (IgG) to Human PDX1
Human, Monkey, Rabbit
IHC, IHC - Paraffin, ELISA
Unconjugated
Anti-PDX1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3086 concentration 5 ug/ml.
Anti-PDX1 Antibody (phospho-Thr11) LS-C97137
Rabbit Polyclonal to Human PDX1
Human
Dot Blot
Unconjugated
Dot blot of anti-Phospho-IPF-pT11 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Anti-PDX1 Antibody (clone 3H3) LS-C340255
Mouse Monoclonal [clone 3H3] (IgG2a) to Human PDX1
Human
IHC, IHC - Paraffin
Unconjugated
IHC of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
Anti-PDX1 Antibody (aa1-284, Biotin) LS-C689046
Rabbit Polyclonal (IgG) to Mouse PDX1
Mouse, Human, Rat
IHC, Western blot
Biotin Conjugated
Western blot of PDX1 antibody.
Anti-PDX1 Antibody (aa1-284, FITC) LS-C696106
Rabbit Polyclonal (IgG) to Mouse PDX1
Mouse, Human, Rat
IHC, Western blot
FITC Conjugated
Western blot of PDX1 antibody.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human PDX1
Human, Monkey, Rabbit
IHC, IHC - Paraffin, ELISA
Unconjugated

Details

Human PDX1
Rabbit
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ELISA

Specificity and Use

PDX1 antibody was raised against synthetic peptide, aa198-213 RMKWKKEEDKKRGGGT corresponding to human Pdx1 (KLH). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%); Bat, Opossum (94%); Mouse, Rat, Hamster, Dog (88%); Turkey, Chicken, Xenopus (81%).
aa198-213
Recognizes human Pdx1. Species cross-reactivity: rodent.
Immunohistochemistry: LS-B3086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3086 was determined to be 5 ug/ml.

Packaging

PBS pH 7.2, 0.02% sodium azide, before the addition of glycerol to 40%.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About PDX1

P52945 NM_000209 NP_000200.1

PDX1 Antibody, Glucose-sensitive factor Antibody, Insulin upstream factor 1 Antibody, IPF1 Antibody, Insulin promoter factor 1 Antibody, IPF-1 Antibody, IUF-1 Antibody, STF-1 Antibody, GSF Antibody, IDX-1 Antibody, Islet/duodenum homeobox-1 Antibody, IUF1 Antibody, MODY4 Antibody, PDX-1 Antibody

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.

Immunohistochemistry

Anti-PDX1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3086 concentration 5 ug/ml.
Anti-PDX1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3086 concentration 5 ug/ml.

Requested From: 
Date Requested: 5/25/2018

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