LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PDLIM5 / LIM Antibody (N‑Terminus) LS‑C16212 Best Seller
LIM antibody LS-C16212 is an unconjugated rabbit polyclonal antibody to LIM (PDLIM5) from human, mouse and rat. Validated for WB.
Antibody
Catalog
Size
Price
LS-C16212-100
100 µg (0.25 mg/ml)
$605

Popular PDLIM5 / LIM Products

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.
Species: Human
Applications: Western blot, Flow Cytometry
Anti-PDLIM5 / LIM antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11243 dilution 1:100.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded Mouse heart.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot

Product Description

LIM antibody LS-C16212 is an unconjugated rabbit polyclonal antibody to LIM (PDLIM5) from human, mouse and rat. Validated for WB.
About PDLIM5 / LIM
May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses. Q96HC4 NM_006457 NP_006448.4

PDLIM5 Antibody, ENH1 Antibody, L9 Antibody, PDZ and LIM domain protein 5 Antibody, Lim Antibody, PDZ and LIM domain 5 Antibody, ENH Antibody, Enigma homolog Antibody, Enigma-like LIM domain protein Antibody


Specifications

Target
Human PDLIM5 / LIM
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PDLIM5 / LIM antibody was raised against synthetic peptide derived from the N-terminal region of the human ENH (enigma homologue 1, LIM protein, protein kinase C- binding protein enigma) protein, which differs from mouse and rat ENH by one amino acid.
Epitope
N-Terminus
Specificity
Recognizes human ENH proteins. Reactivity has been confirmed with human PC-3, LNCap-FGC, Caco-2, and HT-29 cell lysates, mouse heart and skeletal muscle homogenates and rat heart homogenates.
Usage
Suitable for use in Western Blot. Western Blot: 1-3 ug/ml; Target band at ~65kD.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 8/17/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number