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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDK3 Antibody (clone 2B11) LS‑C197867
PDK3 antibody LS-C197867 is an unconjugated mouse monoclonal antibody to human PDK3. Validated for ELISA and WB.
Catalog
Size
Price
LS-C197867-100
100 µg
Unavailable

Popular PDK3 Products

Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus
Applications: Western blot
PDK3 antibody LS-C111083 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Chicken, Zebrafish
Applications: Western blot
Western blot of PDK3 Antibody in MCF-7 cell line lysates (35 ug/lane). PDK3 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Mouse
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

PDK3 antibody LS-C197867 is an unconjugated mouse monoclonal antibody to human PDK3. Validated for ELISA and WB.
About PDK3
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. Q15120 NM_005391 NP_005382.1

PDK3 Antibody, PDHK3 Antibody


Specifications

Target
Human PDK3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2B11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
PDK3 antibody was raised against pDK3 (AAH15948, aa174-263 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PDK3
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

Western blot

PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.
PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

Western blot

PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.
PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

Western blot

PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.
PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.

Western blot

Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

Western blot

PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.
PDK3 monoclonal antibody (M01), clone 2B11 Western Blot analysis of PDK3 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PDK3 is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/22/2018

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