Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 38H8] (IgG1,k) to Yeast PDI1p / Protein Disulfide Isomerase
Immunofluorescence, Western blot
Yeast PDI1p / Protein Disulfide Isomerase
Yeast (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [38H8]
Tissue culture supernatant
Immunofluorescence (1:500 - 1:5000)
Western blot (1:5000 - 1:10000)
Specificity and Use
Yeast nuclear preparations
Western blots of yeast protein extracts probed with Protein Disulfide Isomerase 1p (PDI1p) antibody diluted 1:20000 (ECL detection) reveal a ~65 kDa band. Initial dilution range: 1:5000-1:10000. Immunofluorescence using yeast cells with the antibody gives an ER staining pattern, and for this purpose should be used at a dilution of 1:500-1:5000.
Tissue culture supernatant, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.