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Anti-PDGF-CC Antibody (aa230-345) LS-C150028

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PDGF-CC Antibodies

Anti-PDGF-CC Antibody (aa230-345) LS-C150028
Goat Polyclonal (IgG) to Human PDGF-CC
Human
IHC - Paraffin, Western blot, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-PDGF-CC Antibody LS-C150225
Goat Polyclonal (IgG) to Mouse PDGF-CC
Mouse
IHC, Western blot, ELISA
Unconjugated
Anti-PDGF-CC Antibody (aa23-345, Biotin) LS-C300439
Rabbit Polyclonal (IgG) to Mouse PDGF-CC
Mouse
Western blot, ELISA
Biotin Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human PDGF-CC
Human
IHC - Paraffin, Western blot, Neutralization
Unconjugated

Details

Human PDGF-CC
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Neutralization

Specificity and Use

PDGF-CC antibody was raised against e. coli-derived recombinant human PDGFC Gly230-Gly345 Accession # Q9-NRA1.
aa230-345
Detects human PDGFC in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse.
Western Blot, 1 µg/ml. Immunohistochemistry, 5-15 µg/ml. Neutralization, Measured by its ability to neutralize PDGF-CC-induced proliferation in the NR6R-3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 6-24 µg/ml in the presence of 0.8 µg/ml Recombinant Human PDGF-CC.

Packaging

Lyophilized from PBS, pH 7.4, 5% trehalose
Reconstitute with 500ul sterile PBS
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About PDGF-CC

Q9NRA1 NM_016205 NP_057289.1

PDGFC Antibody, FALLOTEIN Antibody, SCDGF Antibody, VEGF-E Antibody, PDGF-C Antibody

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development.

Immunohistochemistry

Immunohistochemistry
PDGF-C in Human Pancreatic Cancer Tissue. PDGF-C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 ug/ml Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human PDGFC by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human PDGFC Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for PDGF Cp80 atapproximately 80 kD and PDGFC p55 at approximately 55 kD (as indicated). This experiment was conducted under reducing conditions.

Neutralization

Neutralization
Cell Proliferation Induced by PDGF-CC and Neutralization by Human PDGF-C Antibody. Recombinant Human PDGF-CC stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 ug/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 ug/ml.
 

Requested From: United States
Date Requested: 12/4/2016

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