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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDGF‑BB Antibody (aa212‑241) LS‑C97872
PDGF-BB antibody LS-C97872 is an unconjugated rabbit polyclonal antibody to human PDGF-BB. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C97872-400
400 µl
Unavailable

Popular PDGF-BB Products

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Product Description

PDGF-BB antibody LS-C97872 is an unconjugated rabbit polyclonal antibody to human PDGF-BB. Validated for ICC, IF and WB.
About PDGF-BB
PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. P01127 NM_002608 NP_002599.1

PDGFB Antibody, Becaplermin Antibody, PDGF-BB Antibody, PDGF-b Antibody, SSV Antibody, PDGF subunit B Antibody, PDGF-2 Antibody, PDGF2 Antibody, Proto-oncogene c-Sis Antibody, SIS Antibody, C-sis Antibody, PDGF, B chain Antibody


Specifications

Target
Human PDGF-BB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E4839 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97872. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa212-241
Specificity
This PDGFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the C-terminal region of human PDGFB.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.
The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.

Immunofluorescence

Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.
The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.

Immunofluorescence

Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.
The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.

Immunofluorescence

Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of PDGFB Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.
The anti-PDGFB C-term antibody is used in Western blot to detect PDGFB in A375 cell lysate.


Requested From: 
Date Requested: 6/20/2018

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