Target
Mouse PCOLCE
Synonyms
PCOLCE | PCPE | PCPE-1 | PCPE1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived rmPCPE-1.
Specificity
Selected for its ability to recognize mouse PCPE-1 in direct ELISAs and Western Blots. In these formats, this antibody shows ~35% cross-reactivity with rhPCPE.
Applications
- Western blot
- Immunoprecipitation
- ELISA
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse PCPE-1. The detection limit for rmPCPE-1 is approximately 1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rmPCPE-1 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse PCPE-1. The detection limit for rmPCPE-1 is approximately 0.5 ng/well.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 500 ul of Sterile PBS
Storage
Store at -20°C lyophilized. Once reconstituted: Store at -20°C. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee