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Anti-Paraplegin / SPG7 Antibody (clone 7D10) LS-C175084

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Paraplegin / SPG7 Antibodies

Anti-Paraplegin / SPG7 Antibody (aa115-141) LS-C161447
Rabbit Polyclonal to Human Paraplegin / SPG7
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Paraplegin / SPG7 Antibody (aa115-141, Biotin) LS-C270183
Rabbit Polyclonal (IgG) to Human Paraplegin / SPG7
Human
IHC, Western blot, ELISA
Biotin Conjugated
Anti-Paraplegin / SPG7 Antibody (aa115-141, PE) LS-C270186
Rabbit Polyclonal (IgG) to Human Paraplegin / SPG7
Human
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7D10] (IgG1) to Human Paraplegin / SPG7
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human Paraplegin / SPG7
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [7D10]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

Paraplegin / SPG7 antibody was raised against human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 (NP_003110) produced in E. coli.
Human Paraplegin / SPG7

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About Paraplegin / SPG7

Q9UQ90 NM_003119 NP_003110.1

SPG7 Antibody, Cell adhesion regulator Antibody, Cell matrix adhesion regulator Antibody, Paraplegin Antibody, PGN Antibody, SPG5C Antibody, Spastic paraplegia 7 protein Antibody, CAR Antibody, CMAR Antibody

Paraplegin / SPG7 is a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.

Immunofluorescence

Immunofluorescence
Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPG7 antibody, and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/8/2016

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