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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAK4 Antibody (N‑Terminus) LS‑C9450
PAK4 antibody LS-C9450 is an unconjugated rabbit polyclonal antibody to PAK4 from human, mouse, rat and other species. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C9450-100
100 µl
$530

Popular PAK4 Products

Detection of Human PAK4 by Western Blot following Immunoprecipitation. Samples: Whole cell lysate from HeLa (5 x 106) or Jurkat (25 x 106) cells. Antibody: Affinity purified rabbit anti-PAK4 used at 4 ug for IP and at 1 ug/ml for Western Blot. Detection: Chemiluminescence.
Species: Human, Dog, Guinea pig, Pig, Rabbit
Applications: Western blot, Immunoprecipitation
Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine
Applications: ICC, Immunofluorescence, Western blot
Anti-PAK4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B538 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded rat kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

PAK4 antibody LS-C9450 is an unconjugated rabbit polyclonal antibody to PAK4 from human, mouse, rat and other species. Validated for IP and WB.

Specifications

Target
Human PAK4
Synonyms
PAK4, KIAA1142, p21(CDKN1A)-activated kinase 4, PAK-4, p21-activated kinase 4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
Immunogen
PAK4 antibody was raised against synthetic peptide corresponding to the N-terminal residues of human PAK4 (KLH coupled).
Epitope
N-Terminus
Specificity
Detects endogenous levels of PAK4. (MW 72kD) Does not cross-react with other PAKs. Species cross-reactivity: Human, mouse, rat and monkey.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PAK4
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. O96013 NM_005884 NP_005875.1


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Requested From: United States
Date Requested: 10/15/2018

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