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Anti-PAK4 Antibody (N-Terminus) LS-C9450

Catalog Size Price
LS-C9450-100 100 µl Unavailable

Related Products

52 PAK4 Antibodies

Most Popular PAK4 Antibodies

Anti-PAK4 Antibody (Internal) IHC-plus™ LS-B538
Rabbit Polyclonal (IgG) to Human PAK4
Human
IHC - Paraffin, Western blot
Unconjugated
Anti-PAK4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B538 concentration 5 ug/ml.
Anti-PAK4 Antibody (phospho-Ser474) LS-C47727
Rabbit Polyclonal to Human PAK4
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Anti-PAK4 Antibody (aa25-75) LS-C287254
Rabbit Polyclonal (IgG) to Human PAK4
Human, Dog, Guinea pig, Pig, Rabbit
Western blot, Immunoprecipitation
Unconjugated
Detection of Human PAK4 by Western Blot following Immunoprecipitation. Samples: Whole cell lysate from HeLa (5 x 106) or Jurkat (25 x 106) cells. Antibody: Affinity purified rabbit anti-PAK4 used at 4 ug for IP and at 1 ug/ml for Western Blot. Detection: Chemiluminescence.
Anti-PAK4 Antibody LS-C332245
Rabbit Polyclonal (IgG) to Human PAK4
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Immunohistochemistry of paraffin-embedded human tonsil tissue.
Anti-PAK4 Antibody (phospho-Ser474) LS-C353257
Rabbit Polyclonal to Human PAK4
Human, Mouse, Rat, Bovine
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

100% Guaranteed
Rabbit Polyclonal (IgG) to Human PAK4
Human, Monkey, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated

Details

Human PAK4
Rabbit
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000)
  • Immunoprecipitation (1:50)

Specificity and Use

PAK4 antibody was raised against synthetic peptide corresponding to the N-terminal residues of human PAK4 (KLH coupled).
N-Terminus
Detects endogenous levels of PAK4. (MW 72kD) Does not cross-react with other PAKs. Species cross-reactivity: Human, mouse, rat and monkey.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50.

Packaging

10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PAK4

O96013 NM_005884 NP_005875.1

PAK4 Antibody, KIAA1142 Antibody, p21(CDKN1A)-activated kinase 4 Antibody, PAK-4 Antibody, p21-activated kinase 4 Antibody

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues.

Requested From: 
Date Requested: 4/20/2018

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