Target
Human PAK1 + PAK2 + PAK3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Human PAK 1/2/3 phospho-peptide corresponding to a region of the human protein conjugated to KLH. Sequence Information: PEQSKRS-(pT)-MVGTPY (aa416-429 of human PAK1, aa395-408 of human PAK2 and aa429-444 of human PAK3).
Epitope
pThr423/Thr402/Thr436
Specificity
Specific for the phosphorylated form of PAK 1/2/3. Minimally reactive with the non-phosphorylated form of the immunizing peptide. Reactivity with non-phosphorylated PAK 1/2/3 is less than 1% by ELISA.
Usage
Suitable for use in ELISA. Recommended Dilution (s): ELISA: 1:5000-1:25000; Assayed against 0.1 ug of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at RT. Positive control: Lysates from Jurkat cells or PAK transfected cells may be used as a control.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee