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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PAK1 + PAK2 + PAK3 Antibody (phospho‑Thr423) LS‑C18892
PAK1 + PAK2 + PAK3 antibody LS-C18892 is an unconjugated rabbit polyclonal antibody to human PAK1 + PAK2 + PAK3. Validated for ELISA.
Catalog
Size
Price
LS-C18892-100
100 µg
$425
Description
PAK1 + PAK2 + PAK3 antibody LS-C18892 is an unconjugated rabbit polyclonal antibody to human PAK1 + PAK2 + PAK3. Validated for ELISA.
Target
Human PAK1 + PAK2 + PAK3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ELISA (1:5000 - 1:25000)
Epitope
pThr423
Specificity
Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immunoprecipitation. Lysates from Jurkat cells or PAK transfected cells may be used as a control. This product has been assayed against 0.1 ug of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) catalog no. TMBE-100 as a substrate for 30 minutes at room temperature. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Researchers should determine optimal titers for other applications.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% (w/v) Sodium Azide
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.

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Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Ab-423/402/421) antibody (left) or the same antibody preincubated with blocking peptide (right).
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Immunohistochemistry analysis of paraffin-embedded human brain, using PAK1/2/3 (Phospho-Ser144/141/139) Antibody. The picture on the right is blocked with the phospho peptide.
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Requested From: United States
Date Requested: 12/13/2018
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