LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PAK1 Antibody (phospho‑Thr212) LS‑C199399
PAK1 antibody LS-C199399 is an unconjugated rabbit polyclonal antibody to PAK1 from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199399-50
50 µl
Unavailable
LS-C199399-100
100 µl
Unavailable

Popular PAK1 Products

Antibody (1 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-PAK1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4853 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-PAK1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6113 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PAK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7619 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PAK1 antibody LS-C199399 is an unconjugated rabbit polyclonal antibody to PAK1 from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About PAK1
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Q13153 NM_002576 NP_002567.3

PAK1 Antibody, Alpha-PAK Antibody, p21-activated kinase 1 Antibody, p65-PAK Antibody, Rac/p21-activated kinase Antibody, STE20 homolog, yeast Antibody, PAKalpha Antibody, PAK-1 Antibody


Specifications

Target
Human PAK1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PAK1 antibody was raised against synthetic peptide derived from human PAK1 around the phosphorylation site of Thr212.
Epitope
pThr212
Specificity
PAK1 (Phospho-Thr212) Antibody detects endogenous levels of PAK1 only when phosphorylated at threonine212.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/20/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number