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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PH / PAH Antibody (C‑Terminus) IHC‑plus™ LS‑B13580
Note: This antibody replaces LS-C352616
PAH antibody LS-B13580 is an unconjugated rabbit polyclonal antibody to PAH (PH) from human, mouse and bovine. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B13580-50
50 µl (1 mg/ml)
$425

Popular PH / PAH Products

Anti-PH / PAH antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Bat, Dog, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: Western blot
Western blot of recombinant PH / PAH.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Pig
Applications: IHC, Western blot
Immunohistochemical of paraffin-embedded M. kidney section using PAH Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PH / PAH Antibody (C-Terminus) for IHC, WB/Western IHC-plus™ LS-B13580

Specifications

Target
PH / PAH
Synonyms
PAH, Phe-4-monooxygenase, PKU1, PH, Phenylalanine 4-monooxygenase, Phe-4- monooxygenase, Phenylalanine hydroxylase, Phenylalanine-4-hydroxylase, PKU
Host
Rabbit
Reactivity
Human, Mouse, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PH / PAH antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human PAH.
Blocking Peptide
LS-E42029 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-B13580. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of PAH protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About PH / PAH
PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. P00439 NM_000277 NP_000268.1


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Images

Immunohistochemistry - Paraffin

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.
Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.

Immunohistochemistry - Paraffin

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.
Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.

Immunohistochemistry - Paraffin

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.
Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.

Immunohistochemistry - Paraffin

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.
Western blot analysis of PAH expression in HepG2 (A); mouse liver (B) whole cell lysates.

Requested From: United States
Date Requested: 10/22/2018

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