(tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine
Protein A purified
PAG1 antibody was raised against recombinant intracellular fragment (amino acids 97-432) of human Cbp/PAG.
Recognizes the transmembrane adaptor protein known as Protein Associated with Glycosphingolipid-enriched microdomains (PAG), an epitope (aa 235-280) located in the cytoplasmic domain of csk-binding protein (Cbp), which localizes in membrane lipid rafts. The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs). Evidence has shown that by binding to the SH2 domain of the PTK csk, a major negative regulator of src-family kinases, PAG acts not only acts as a co-factor for relocating csk to substrate rich microdomains, but also increases the affinity of csk for src-family kinases, through the formation of PAG-csk complexes.
Western Blot: LS-C44850 detects a band of approximately 50 kD in Ramos cell lysates.
PBS, pH 7.4, 0.09% sodium azide.
For research use only.
This antibody carries the LSBio 100% Guarantee.