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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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P5CR2 / PYCR2 Antibody (clone 2F11) LS‑C115756
PYCR2 antibody LS-C115756 is an unconjugated mouse monoclonal antibody to PYCR2 (P5CR2). Validated for IF and WB.
Catalog
Size
Price
LS-C115756-100
100 µl
Unavailable

Popular P5CR2 / PYCR2 Products

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
Applications: Western blot
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Applications: Western blot
Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Species: Human, Monkey
Applications: Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Human colon tissue using anti-PYCR2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

PYCR2 antibody LS-C115756 is an unconjugated mouse monoclonal antibody to PYCR2 (P5CR2). Validated for IF and WB.
About P5CR2 / PYCR2
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP+. Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Q96C36 NM_013328 NP_037460.2

PYCR2 Antibody, p5CR 2 Antibody, p5C reductase 2 Antibody, p5CR2 Antibody


Specifications

Target
Human P5CR2 / PYCR2
Host
Mouse
Clonality
IgG2b Monoclonal [2F11]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
Immunogen
P5CR2 / PYCR2 antibody was raised against full length human recombinant protein of human PYCR2 (NP_037460) produced in HEK293T cell
Specificity
Human PYCR2
Usage
Concentration: 1.00 mg/ml (Lot dependent)
Presentation
PBS (PH 7.3), 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.

Immunofluorescence

Anti-PYCR2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.
Anti-PYCR2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCR2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCR2.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCR2 monoclonal antibody.


Requested From: 
Date Requested: 6/21/2018

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