Target
Human p56lck / LCK
Synonyms
LCK | Lck kinase | LSK | p56-LCK | Pp58lck | Tyrosine-protein kinase Lck | p56lck | Protein YT16 | Proto-oncogene Lck | YT16
Reactivity
Human, Monkey, Mouse, Bovine, Horse, Rabbit, Sheep
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RNGSEVRDPLVTYE, from the internal region (near N-Terminus) of the protein sequence according to NP_005347.3.
Specificity
Human LCK. This antibody is not expected to cross-react with other tyrosine protein kinases. Reported variants represent identical protein: NP_005347.3, NP_001036236.1
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 60kD band observed in Human Thymus lysates (calculated MW of 58.0kD according to NP_005347.3). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee