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Anti-P40PHOX / NCF4 Antibody (aa228-238) IHC-plus™ LS-B3604

Note: This antibody replaces LS-C55151

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular P40PHOX / NCF4 Antibodies

Anti-P40PHOX / NCF4 Antibody IHC-plus™ LS-B2624
Rabbit Polyclonal (IgG) to Mouse P40PHOX / NCF4
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-P40PHOX / NCF4 Antibody (aa228-238) IHC-plus™ LS-B3604
Goat Polyclonal to Human P40PHOX / NCF4
Human, Monkey, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human P40PHOX / NCF4
Human, Monkey, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human P40PHOX / NCF4
Goat
Human, Monkey, Horse (tested or 100% immunogen sequence identity)
Bovine, Dog, Hamster, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:32000) & (0.3 - 13 µg/ml)
  • ELISA (1:32000)

Specificity and Use

P40PHOX / NCF4 antibody was raised against synthetic peptide C-KDFPEEDDPTN from an internal region of human NCF4 (NP_000622.2; NP_038202.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%); Hamster, Panda, Dog, Bovine, Pig (91%); Drosophila, Mosquito, Sea anemone, Ant, Water flea, Beetle, Tick (82%).
LS-E26389 - Lyophilized - 100 µg - $145.00
aa228-238
Human NCF4. This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Immunohistochemistry: LS-B3604 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3604 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About P40PHOX / NCF4

Q15080 NM_013416 NP_038202.2

NCF4 Antibody, SH3PXD4 Antibody, NCF Antibody, Neutrophil cytosol factor 4 Antibody, p40-phox Antibody, p40PHOX Antibody, NCF-4 Antibody

P40PHOX / NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation.

Immunohistochemistry

Immunohistochemistry
Anti-NCF4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3604 concentration 2.5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-NCF4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3604 concentration 2.5 ug/ml.

Western blot

Western blot
Antibody (0.5 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/7/2016

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