Human OPRM1 / Mu Opioid Receptor
Human, Monkey, Mouse, Rat, Horse, Pig
(tested or 100% immunogen sequence identity)
Bovine, Dog, Rabbit (at least 90% immunogen sequence identity)
- IHC - Frozen
- Western blot
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Synthetic peptide corresponding to aa384-398 (NHQLENLEAETAPLP) from the C-terminus of the cloned rat mu opioid receptor-1 (bovine thyroglobulin coupled by glutaraldehyde). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Horse, Pig, Opossum (100%); Bovine, Dog, Rabbit, Platypus (93%); Turkey, Chicken (80%).
Mu opioid receptor, C-terminal domain. Specificity confirmed by immunodot-blot assay. No reactivity to the N-terminus, second extracellular loop or a different segment of the carboxy tail of the mu opioid receptor. No reactivity to either the delta or kappa opioid receptor. Species cross-reactivity: Human, rat and mouse.
Suitable for use in Immunofluorescence, Immunohistochemistry, and Western Blot. Immunohistochemistry: 1:500-1:1000 (Cy3 technique); 1:6000-1:10000 (Biotin/avidin-HRP technique). Dilute antibody in PBS, 0.3% Triton X-100. Incubate 18-24 hrs at 2-8?C. Perfusion Fixation: With 500 ml 0.1 M phosphate buffer, pH 7.4, 4% paraformaldehyde for 20-30 minutes. Post Fixation: 0.1 M phosphate buffer, pH 7.4, 4% paraformaldehyde for 1.5 hours at 4?C. Sections: 10um cryostat. Note that a change in the fixation or buffering system as used in our protocol may change the configuration of the protein, and therefore, may alter the reactivity with the tissue tested. Pre-adsorption with immunogen peptide at 10 ug/ml completely eliminates labeling.
0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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