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Anti-OLFM4 / Olfactomedin 4 Antibody (aa400-450) IHC-plus™ LS-B2055

Note: This antibody replaces LS-C47338
Catalog Size Price
LS-B2055-50 50 µg (0.5 mg/ml) Unavailable

Most Popular OLFM4 / Olfactomedin 4 Antibodies

Anti-OLFM4 / Olfactomedin 4 Antibody (N-Terminus) LS-C47070
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human
Western blot, ELISA
Unconjugated
Anti-OLFM4 / Olfactomedin 4 Antibody (aa400-450) IHC-plus™ LS-B2055
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ LS-A9926
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ LS-A8454
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ LS-A8455
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human, Dog
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human OLFM4 / Olfactomedin 4
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human OLFM4 / Olfactomedin 4
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)

Specificity and Use

OLFM4 / Olfactomedin 4 antibody was raised against a synthetic peptide corresponding to amino acids 400-419 (YVYNDGYLLNYDLSVLQKPQ) of human OLFM4 was used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Marmoset (95%); Monkey, Mouse, Rat (85%).
aa400-450
A portion of amino acids 400-450 of human Olfactomedin 4.
Immunohistochemistry: LS-B2055 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2055 was determined to be 5 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About OLFM4 / Olfactomedin 4

Q6UX06 NM_006418 NP_006409.3

OLFM4 Antibody, Antiapoptotic protein GW112 Antibody, HOLfD Antibody, HGC-1 Antibody, GW112 Antibody, OlfD Antibody, OLM4 Antibody, UNQ362 Antibody, Olfactomedin 4 Antibody, Olfactomedin-4 Antibody, BA209J19.1 Antibody, GC1 Antibody, HCG-1 Antibody

Olfactomedin 4 (OLFM4) was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.

Immunohistochemistry

Immunohistochemistry
Anti-OLFM4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2055 concentration 5 ug/ml.

Western blot

Western blot
Western blot of human Olfactomedin 4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using LS-B2055 at 2 ug/ml.

Requested From: 
Date Requested: 6/28/2017

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