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Anti-OGT / O-GLCNAC Antibody (aa223-502) LS-C186114

Catalog Size Price
LS-C186114-100 100 µl (1 mg/ml) Unavailable

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21 OGT / O-GLCNAC Antibodies

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Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-OGT / O-GLCNAC Antibody (aa543-555) IHC-plus™ LS-B2534
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Anti-OGT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2534 concentration 5 ug/ml.
Anti-OGT / O-GLCNAC Antibody (aa223-502) LS-C186114
Rabbit Polyclonal (IgG) to Human OGT / O-GLCNAC
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IHC, IHC - Paraffin, ICC, Western blot
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IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Anti-OGT / O-GLCNAC Antibody IHC-plus™ LS-B13864
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Immunohistochemistry of paraffin-embedded rat lung using OGT antibody at dilution of 1:100 (400x lens).
Anti-OGT / O-GLCNAC Antibody LS-C482338
Rabbit Polyclonal to Human OGT / O-GLCNAC
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IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
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Immunohistochemical analysis of OGT staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human OGT / O-GLCNAC
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human OGT / O-GLCNAC
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

OGT / O-GLCNAC antibody was raised against recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (SwissProt O15294).
aa223-502
Human OGT / O-GLCNAC
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About OGT / O-GLCNAC

O15294 U77413 AAB63466.1

OGT Antibody, HRNT1 Antibody, O-GLCNAC Antibody

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.

Western blot

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000

Western blot

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.

Requested From: 
Date Requested: 4/25/2018

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