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OGT / O‑GLCNAC Antibody (aa223‑502) LS‑C186114
O-GLCNAC antibody LS-C186114 is an unconjugated rabbit polyclonal antibody to human O-GLCNAC (OGT). Validated for ICC, IHC and WB.
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Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications:
Western blot
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product Description
O-GLCNAC antibody LS-C186114 is an unconjugated rabbit polyclonal antibody to human O-GLCNAC (OGT). Validated for ICC, IHC and WB.
About OGT / O-GLCNAC
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
O15294
U77413
AAB63466.1
OGT Antibody, HRNT1 Antibody, O-GLCNAC Antibody
Specifications
Target
Human OGT / O-GLCNAC
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (1:100 - 1:1000)
- ICC (1:100 - 1:1000)
- Western blot (1:500 - 1:3000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Immunogen
OGT / O-GLCNAC antibody was raised against recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (SwissProt O15294).
Epitope
aa223-502
Specificity
Human OGT / O-GLCNAC
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Western blot
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
Western blot
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Western blot
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
Western blot
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Western blot
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
Western blot
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Western blot
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
Western blot
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Requested From:
Date Requested: 6/22/2018
Date Requested: 6/22/2018
