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TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Rabbit Polyclonal (IgG) to Human OGT / O-GLCNAC
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
Details
Human OGT / O-GLCNAC
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified
Applications
IHC
IHC - Paraffin (1:100 - 1:1000)
ICC (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
OGT / O-GLCNAC antibody was raised against recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (SwissProt O15294).
aa223-502
Human OGT / O-GLCNAC
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Packaging
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Western blot
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
Western blot
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.