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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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OGT / O‑GLCNAC Antibody (aa223‑502) LS‑C186114
O-GLCNAC antibody LS-C186114 is an unconjugated rabbit polyclonal antibody to human O-GLCNAC (OGT). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186114-100
100 µl (1 mg/ml)
Unavailable

Popular OGT / O-GLCNAC Products

Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
Anti-OGT antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2534 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry of paraffin-embedded rat lung using OGT antibody at dilution of 1:100 (400x lens).
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemical analysis of OGT staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

O-GLCNAC antibody LS-C186114 is an unconjugated rabbit polyclonal antibody to human O-GLCNAC (OGT). Validated for ICC, IHC and WB.
About OGT / O-GLCNAC
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. O15294 U77413 AAB63466.1

OGT Antibody, HRNT1 Antibody, O-GLCNAC Antibody


Specifications

Target
Human OGT / O-GLCNAC
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
OGT / O-GLCNAC antibody was raised against recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (SwissProt O15294).
Epitope
aa223-502
Specificity
Human OGT / O-GLCNAC
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.

Western blot

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000

Western blot

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.

Western blot

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000

Western blot

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.

Western blot

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000

Western blot

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using O-GlcNAc transferase antibody at 1:200 dilution.

Western blot

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000
O-GlcNAc transferase antibody detects OGT protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. O-GlcNAc transferase antibody dilution:1:1000

Western blot

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. OGT / O-GLCNAC antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/22/2018

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