Human NUMB

Sheep

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

E. coli-derived recombinant human rhNumb.

Recognizes human Numb. Species sequence homology: mouse: 88% and canine: 90%.

• ICC (10 µg/ml)
• Western blot (1 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western blot, Immunocytochemistry, and Direct ELISA. Western blot: 1.0 ug/ml. Immunocytochemistry: 10 ug/ml Fix cells with PBS containing 4% paraformaldehyde for 20 minutes at RT and block with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. Follow by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~0.1 ng/well.

Lyophilized from PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49757 NCBI: NM_003744 NP_003735.3