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Anti-NRF1 / NRF-1 Antibody (clone 2F9) LS-C105005

Catalog Size Price
LS-C105005-100 100 µg Unavailable

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36 NRF1 / NRF-1 Antibodies

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100% Guaranteed
Mouse Monoclonal [clone 2F9] (IgG1,k) to Human NRF1 / NRF-1
Human
Western blot, ELISA, RNA interference
Unconjugated

Details

Human NRF1 / NRF-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2F9]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA
  • RNA interference

  • IHC - Paraffin

Specificity and Use

NRF1 / NRF-1 antibody was raised against nRF1 (NP_005002, 201 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Tissue lysate) - positive control human Skeletal muscle. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human kidney. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Packaging

PBS, pH 7.2
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About NRF1 / NRF-1

Q16656 NM_005011 NP_005002.3

NRF1 Antibody, EWG Antibody, NRF-1 Antibody, Nuclear respiratory factor 1 Antibody, ALPHA-PAL Antibody

NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene.

Western blot

Western blot
NRF1 monoclonal antibody (M01), clone 2F9. Western blot of NRF1 expression in human Skeletal muscle.

Western blot

Western blot
Western blot of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NRF1 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody, clone 2F9. GAPDH ( 36.1 kD ) used as specificity and.

Requested From: 
Date Requested: 8/21/2017

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