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Anti-NRF1 / NRF-1 Antibody (aa1-534) IHC-plus™ LS-B43

Note: This antibody replaces LS-C18854


Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

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Anti-NRF1 / NRF-1 Antibody (aa1-534) IHC-plus™ LS-B43
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Immunohistochemistry Image
Anti-NRF1 / NRF-1 Antibody (aa429-478) LS-C31808
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Anti-NRF1 / NRF-1 Antibody (aa360-409) IHC-plus™ LS-B3511
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Anti-NRF1 / NRF-1 Antibody (N-Terminus) IHC-plus™ LS-B11495
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Anti-NRF1 / NRF-1 Antibody IHC-plus™ LS-B12877
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Rabbit Polyclonal to Mouse NRF1 / NRF-1
Mouse, Human
IHC - Paraffin, Western blot, ELISA


Mouse NRF1 / NRF-1
Mouse, Human (tested or 100% immunogen sequence identity)
Protein A purified


  • IHC - Paraffin (2 - 5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:3000 - 1:10000)

Specificity and Use

NRF1 / NRF-1 antibody was raised against recombinant mouse NRF1.
Purified recombinant mouse NRF1 protein corresponding to aa 1-534 of the native protein.
Immunohistochemistry: LS-B43 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B43 was determined to be 2-5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NRF1 / NRF-1

Q16656 NM_005011 NP_005002.3

NRF1 Antibody, EWG Antibody, NRF-1 Antibody, Nuclear respiratory factor 1 Antibody, ALPHA-PAL Antibody

NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene.


Anti-NRF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B43 concentration 5 ug/ml.

Western blot

Western blot
Anti-NRF1 Antibody - Western Blot. Western blot of Protein A Purified anti-NRF1 antibody shows detection of a 67-kD band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye700 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:2500. IRDye700 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 4/22/2017

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