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Anti-NR1I2 / PXR Antibody (aa406-420) IHC-plus™ LS-B3968

Note: This antibody replaces LS-C87400

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Related Products

48 NR1I2 / PXR Antibodies

Most Popular NR1I2 / PXR Antibodies

Anti-NR1I2 / PXR Antibody (C-Terminus) LS-C31660
Rabbit Polyclonal (IgG) to Human NR1I2 / PXR
Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Pig
Western blot
Unconjugated
Western blot Image
Anti-NR1I2 / PXR Antibody (aa108-157) IHC-plus™ LS-B3516
Rabbit Polyclonal (IgG) to Human NR1I2 / PXR
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NR1I2 / PXR Antibody (aa406-420) IHC-plus™ LS-B3968
Goat Polyclonal to Human NR1I2 / PXR
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NR1I2 / PXR Antibody (aa100-127) LS-C162987
Rabbit Polyclonal to Human NR1I2 / PXR
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-NR1I2 / PXR Antibody (aa205-390) LS-C296026
Rabbit Polyclonal (IgG) to Mouse NR1I2 / PXR
Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human NR1I2 / PXR
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human NR1I2 / PXR
Goat
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (1:4000)

Specificity and Use

NR1I2 / PXR antibody was raised against synthetic peptide EQFAITLKSYIECNR from an internal region of human NR1I2 / PXR (NP_003880.3; NP_071285.1; NP_148934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (93%); Horse, Pig (87%); Panda, Dog, Rabbit (80%).
LS-E26983 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3968. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa406-420
Human NR1I2 / PXR. This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 48kD band observed in lysates of cell line HepG2 (calculated MW of 49.8kD according to NP_003880.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About NR1I2 / PXR

O75469 NM_003889 NP_003880.3

NR1I2 Antibody, BXR Antibody, Orphan nuclear receptor PXR Antibody, Orphan nuclear receptor PAR1 Antibody, PARq Antibody, PRR Antibody, PXR Antibody, Pregnane X receptor Antibody, ONR1 Antibody, SAR Antibody, SXR Antibody

Pregnane X receptor (PXR), a NR1 Thyroid Hormone-Like Receptor, has been shown to mediate the effects of steroid hormones, including progesterone, glucocorticoid, and pregnenolone, and effects of xenobiotics, through the cytochrome P-450 3A gene family (CYP3A). PXR protects the body from hepatotoxicity of secondary bile acids such as lithocholic acid (LCA) by inducing expression of the hydroxylating cytochrome P450 enzyme CYP3A and promoting detoxification.

Immunohistochemistry

Immunohistochemistry
Anti-PXR antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3968 concentration 3.75 ug/ml.

Western blot

Western blot
Antibody (1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/24/2017

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