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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NR1D2 Antibody (Internal) IHC‑plus™ LS‑A5645
NR1D2 antibody LS-A5645 is an unconjugated rabbit polyclonal antibody to NR1D2 from human, mouse, rat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A5645-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

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Product Description

NR1D2 antibody LS-A5645 is an unconjugated rabbit polyclonal antibody to NR1D2 from human, mouse, rat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About NR1D2
Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Q14995 D16815 BAA20088.1

NR1D2 Antibody, EAR-1R Antibody, HZF2 Antibody, Nuclear receptor rvr Antibody, Rev-erbabeta Antibody, Rev-erb-beta Antibody, Rev-erba beta Antibody, RVR Antibody, BD73 Antibody, Nuclear receptor ear-1r Antibody


Specifications

Target
Human NR1D2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NR1D2 antibody was raised against synthetic 16 amino acid peptide from internal region of human NR1D2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Panda, Horse, Rabbit, Turkey, Chicken (100%); Elephant, Platypus (94%); Opossum, Xenopus (81%).
Blocking Peptide
LS-E29085 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A5645. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human NR1D2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A5645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A5645 was determined to be 10 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NR1D2 antibody LS-A5645 IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Requested From: 
Date Requested: 6/24/2018

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